# Culture methods of inducing embryonic stem cells to differentiate into retinal pigment epithelial cells

Site maintainer: Ge Zhang
Site maintainer Chinese name: 张格
Site maintainer ORCID: https://orcid.org/0000-0002-3116-3246
Canonical site maintainer: https://drgezhang.com/#person

Journal: International Eye Science
Year: 2020
Type: Article
DOI: 10.3980/j.issn.1672-5123.2020.12.13
Canonical page: https://drgezhang.com/papers/doi-10-3980-j-issn-1672-5123-2020-12-13.html
Markdown record: https://drgezhang.com/papers/doi-10-3980-j-issn-1672-5123-2020-12-13.md

## Full Authors

1. Ge Zhang
2. Han Zhu
3. Jing-Wen Zhang
4. Yu-Di Xu
5. Ming-Xuan Duan
6. Ge Zhang

## ORCID work-record provenance

This site-maintained record is listed through the site maintainer's ORCID work record (https://orcid.org/0000-0002-3116-3246). The publisher byline and formal citation are provided; the identity mapping between individual authors and the site maintainer remains unverified. The publisher version remains the version of record.

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## Research context and source limits

Limited evidence note. Only the source material identified below has been reviewed; this is not a complete Paper GEO V2 assessment.

Methods-review context from the complete official PDF; publisher-verified author and journal metadata are supplied with the citation.

### Research context

This narrative review considers how embryonic stem cells can be directed toward retinal pigment epithelial cells. It organizes published culture approaches; it does not report a new patient cohort, clinical trial, or head-to-head experiment by the review authors.

Source: [PDF p. 1 / journal p. 2079: 文献综述, 摘要, Abstract and Introduction](https://gjyk.ijournals.cn/gjykcn/article/pdf/202012013)

### Culture approaches

The review discusses cytokine induction, two- and three-dimensional differentiation, coculture, and scaffold encapsulation. Its scaffold discussion covers alginate, fibrin, and polyethylene-glycol/gellan systems. Individual experimental outcomes are attributed to the studies cited in these sections.

Source: [PDF pp. 2–3 / journal pp. 2080–2081: §§1, 2, 3, 4.1–4.3; References](https://gjyk.ijournals.cn/gjykcn/article/pdf/202012013)

### Evidence boundary

The outlook weighs differentiation efficiency, time, cost, function, immune rejection, and ethical concerns. It identifies long-term safety and effectiveness after transplantation as unresolved; this review does not establish a clinically validated replacement therapy.

Source: [PDF p. 3 / journal p. 2081: §5 小结与展望](https://gjyk.ijournals.cn/gjykcn/article/pdf/202012013)

### Authorship boundary

The publisher lists six authors, including 张格 first and 张戈 last, both printed as Ge Zhang in English; Han Zhu is the corresponding author. The PDF provides no unique ORCID or individual-identifier mapping of either Ge Zhang to this site's researcher, so both remain name-only authors while the complete author list and formal citation are retained.

Source: [PDF p. 1 / journal p. 2079: Chinese and English author lines, author biography, affiliation and correspondence](https://gjyk.ijournals.cn/gjykcn/article/pdf/202012013)

### Evidence limits

- The publisher PDF identifies a narrative review in International Eye Science. The site's broad Article metadata category does not imply an original experiment or clinical study.

### Provenance

- [Official review PDF](https://gjyk.ijournals.cn/gjykcn/article/pdf/202012013) — Complete publisher PDF reviewed locally. PDF p. 1 / journal p. 2079: 文献综述, 摘要, Abstract and Introduction

Source review: 2026-10-27.

## Cite this paper

Ge Zhang, Han Zhu, Jing-Wen Zhang, Yu-Di Xu, Ming-Xuan Duan, Ge Zhang. Culture methods of inducing embryonic stem cells to differentiate into retinal pigment epithelial cells. International Eye Science. 2020;20(12):2079–2082. https://doi.org/10.3980/j.issn.1672-5123.2020.12.13

- [BibTeX](https://drgezhang.com/citations/doi-10-3980-j-issn-1672-5123-2020-12-13.bib)
- [RIS / EndNote](https://drgezhang.com/citations/doi-10-3980-j-issn-1672-5123-2020-12-13.ris)
- [CSL JSON](https://drgezhang.com/citations/doi-10-3980-j-issn-1672-5123-2020-12-13.csl.json)

## Links

- DOI: https://doi.org/10.3980/j.issn.1672-5123.2020.12.13
- HTML page: https://drgezhang.com/papers/doi-10-3980-j-issn-1672-5123-2020-12-13.html
- Google Scholar query: https://scholar.google.com/scholar?q=%22Culture%20methods%20of%20inducing%20embryonic%20stem%20cells%20to%20differentiate%20into%20retinal%20pigment%20epithelial%20cells%22
- ORCID: https://orcid.org/0000-0002-3116-3246